FastQCFastQC Report
Thu 17 Nov 2022
HMGL2DRX2_l02_n01_RL5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGL2DRX2_l02_n01_RL5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41281771
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC728910.176569459677493TruSeq Adapter, Index 10 (100% over 50bp)
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG676530.16388105054892146No Hit
CTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTAT573730.13897901812400443No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC518860.1256874371983702TruSeq Adapter, Index 10 (98% over 50bp)
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT438410.10619941668684708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)