FastQCFastQC Report
Sat 20 Oct 2018
HMGGHBGX7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGGHBGX7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480537431
Sequences flagged as poor quality0
Sequence length99
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44904420.9344624810299117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA24309580.5058831722933983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG21732420.45225238655758326No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG6777470.14103937722179233No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC5544140.11537373870049261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG17112300.071.385972
AAGCAGT17202600.071.252931
CAGTGGT18550550.065.670734
AGTGGTA18926550.064.429675
GCAGTGG19878250.061.376093
GTGGTAT20034950.060.8982246
GGTATCA20107400.060.6032948
TGGTATC20472450.059.5358357
GTATCAA25943450.047.0688259
CATGGGG14287150.033.59449824-25
AGAGTAC19994150.033.49279818-19
GCAGAGT20245500.033.11207616-17
TACATGG20345700.032.7395122-23
CAACGCA20818350.032.22238512-13
ACGCAGA21106150.031.81841714-15
ATCAACG21133700.031.79798110-11
AGTACAT22235750.030.13040720-21
CAGAGTA20298700.030.09462418-19
CGCAGAG20375550.030.04792416-17
ACATGGG20361550.029.77830724-25
Produced by FastQC (version 0.11.5)