FastQCFastQC Report
Sat 20 Oct 2018
HMGGHBGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGGHBGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480537431
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT1401800.018.4344441
GACTAAC1448400.018.3533361
AGGCGAC717400.018.1067478
GATGAAA1543200.018.0521871
CCACTAC1818200.018.0514621
CTCGAAA1355600.017.948481
CGTGAGC1155900.017.9034121
ACTGAAC1572600.017.8836821
ATAACGC1358400.017.766891
CAATGGA1223450.017.6766828
ACTGATG1253950.017.5706651
GGAATAA1385900.017.5265161
TGGACGC1223900.017.5180341
TTGGAAC1384150.017.4834141
TCTGGAA1529900.017.4690021
TGCGGGT1503050.017.4665871
CTGCGGA935650.017.4657041
ACGAGGA1211750.017.443171
ATAGACC1318950.017.4135281
CCGGTAG1403050.017.4118521
Produced by FastQC (version 0.11.5)