FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21669309
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1519830.7013744646864374No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT269620.12442482591392277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG87900.028.1073445
CTGACTC85450.027.4512862
GCTGACT92500.024.664861
AGCTGAC74750.024.2850931
CTCTAGC101700.024.0421546
TAGCAGA107900.022.9307619
CTAGCAG113150.021.8062218
TCTATCG106950.021.6574916
CTATCGA114250.020.67197617
TATCGAT119500.019.9318618
GATCTAT120800.019.81347714
ATCTATC132500.018.05771815
AGATCTA134700.017.82415813
ATCGATT139750.016.95184119
TCGATTC141950.016.2988120
GACTCTA156500.015.9335474
CAGATCT150950.015.88253312
TTCTAGC148600.015.52876124
TAGCATG155300.015.30854227
CTAGCAT158200.015.02802326
Produced by FastQC (version 0.11.5)