FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21377342
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1109520.519016816964429No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG471780.2206916089006762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG371950.17399263201196857No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA348650.1630932414329153No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA345680.16170391997283853No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA340770.15940709560617966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA210350.040.0324634
ACTCTAG216400.038.9784975
CTGACTC219950.038.268482
GCTGACT226600.037.1767431
TGACTCT231900.036.5535553
CTCTAGC256200.032.9920586
TAGCAGA261500.032.4176869
CTAGCAG272150.031.431888
TCTATCG295750.028.22672316
CTATCGA303650.027.51534317
GATCTAT311800.026.89691214
TATCGAT313850.026.54297418
AGATCTA329000.025.51179113
ATCTATC338950.024.72198315
ATCGATT368600.022.67638219
AGCAGAT382650.022.40106410
CGATTCT372950.022.26172621
TCGATTC380300.021.97858620
CAGATCT388250.021.7897312
TTCTAGC385550.021.5608824
Produced by FastQC (version 0.11.5)