FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21377342
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1109520.519016816964429No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG471780.2206916089006762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG371950.17399263201196857No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA348650.1630932414329153No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA345680.16170391997283853No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA340770.15940709560617966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA210100.039.980184
ACTCTAG215750.039.031075
CTGACTC219100.038.3051532
GCTGACT225900.037.1525341
TGACTCT231300.036.587873
CTCTAGC256150.032.9302026
TAGCAGA260900.032.4251869
CTAGCAG271800.031.4079918
TCTATCG294500.028.26328716
CTATCGA303000.027.50501317
GATCTAT310650.026.92883714
TATCGAT312700.026.57344818
AGATCTA327950.025.52939413
ATCTATC337550.024.7622615
ATCGATT367200.022.6961319
AGCAGAT381350.022.46826610
CGATTCT371350.022.31053221
TCGATTC379500.021.95114720
CAGATCT386400.021.83966812
TTCTAGC384800.021.55744224
Produced by FastQC (version 0.11.5)