FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18113315
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1116190.6162262401995438No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG492300.2717890126683051No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA379970.20977385972694673No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA366490.20233182054196042No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA282050.15571418042473176No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266820.147306001137837No Hit
TCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACC214120.1182113820689366No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG188270.10394011256360308No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT183140.1011079418648657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA200250.040.408684
GCTGACT203300.039.612861
CTGACTC204550.039.387682
ACTCTAG206500.039.2880025
TGACTCT218600.037.1766473
CTCTAGC235750.034.384056
TAGCAGA237200.034.2334489
CTAGCAG250350.032.7708248
TCTATCG288750.027.85591316
CTATCGA292500.027.52256617
GATCTAT299300.027.01435514
TATCGAT306500.026.23108718
AGATCTA309650.026.1227113
ATCTATC314300.025.69168515
AGCAGAT352850.023.370410
CAGATCT360200.022.58294512
ATCGATT356250.022.54828819
CGATTCT359400.022.2727521
TCGATTC370700.021.6693520
TTCTAGC373750.021.44580824
Produced by FastQC (version 0.11.5)