FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18113315
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1116190.6162262401995438No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG492300.2717890126683051No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA379970.20977385972694673No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA366490.20233182054196042No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA282050.15571418042473176No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266820.147306001137837No Hit
TCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACC214120.1182113820689366No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG188270.10394011256360308No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT183140.1011079418648657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA200800.040.5420344
GCTGACT204100.039.680531
CTGACTC205200.039.4846572
ACTCTAG207100.039.4276925
TGACTCT219000.037.3324973
CTCTAGC236450.034.4746976
TAGCAGA238000.034.309579
CTAGCAG250500.032.918888
TCTATCG288950.028.00622716
CTATCGA293200.027.62399517
GATCTAT299600.027.16255614
TATCGAT306850.026.36086818
AGATCTA310300.026.22591613
ATCTATC314550.025.83818615
AGCAGAT353200.023.48598310
CAGATCT361100.022.66236912
ATCGATT356550.022.6569419
CGATTCT360100.022.3655421
TCGATTC371150.021.7751120
TTCTAGC373700.021.56107924
Produced by FastQC (version 0.11.5)