FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47980517
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT2367910.493514898974515No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1461900.30468617084722116No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1200440.2501932190518081No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA732580.15268280664837355No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA675310.14074671183722343No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC524530.10932145645700317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA527000.033.193214
ACTCTAG542050.032.3818785
CTGACTC555050.031.470872
TGACTCT573700.030.7044433
GCTGACT575800.030.4463581
AGCTGAC375050.029.5259081
CTCTAGC617100.028.5465496
TAGCAGA638700.027.587379
CTAGCAG674900.026.4550468
TCTATCG679650.025.50790216
CTATCGA701550.024.71170617
GATCTAT725250.024.0295514
TATCGAT729550.023.83042118
AGATCTA763250.022.86063213
ATCTATC777200.022.47284915
ATCGATT846200.020.57425119
TCGATTC868000.020.0452820
CGATTCT865750.020.03277221
CAGATCT894450.019.70726412
AGCAGAT920450.019.50038110
Produced by FastQC (version 0.11.5)