FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47980517
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT2367910.493514898974515No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1461900.30468617084722116No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1200440.2501932190518081No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA732580.15268280664837355No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA675310.14074671183722343No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC524530.10932145645700317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA530850.033.104094
ACTCTAG545700.032.3320545
CTGACTC558200.031.4814132
TGACTCT574650.030.7633173
GCTGACT577600.030.5211451
AGCTGAC379150.029.5666391
CTCTAGC621100.028.5148056
TAGCAGA642000.027.5763329
CTAGCAG675750.026.5304668
TCTATCG680750.025.57977916
CTATCGA701850.024.81577717
GATCTAT727850.024.03989614
TATCGAT729500.023.94234318
AGATCTA765300.022.90459413
ATCTATC777950.022.52317615
ATCGATT845100.020.70035219
CGATTCT864500.020.17101921
TCGATTC867750.020.16393920
CAGATCT898850.019.68866212
AGCAGAT925750.019.45674310
Produced by FastQC (version 0.11.5)