FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27962279
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT2092980.748501221949756No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1586350.5673178498791175No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG992710.3550175577605817No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA770440.27552832871741245No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA641450.22939832622369585No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC608250.2175251881293367No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC434310.1553199580048536No Hit
CGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACG388070.13878339458668587No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG368330.1317238841655217No Hit
TCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACC330070.11804116538569691No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC312930.11191147903216328No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG310240.11094946874680708No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG301290.10774872820630964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298180.10663651557156696No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG297610.10643266952597105No Hit
GCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATGTACCCCG293910.10510945835280451No Hit
CCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGT285720.10218051253976831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC308350.038.489551
GACTCTA476150.032.202594
ACTCTAG484200.031.667385
CTGACTC484050.031.6257922
GCTGACT492100.031.1231081
TGACTCT498950.030.815073
CTCTAGC543250.028.2707126
TAGCAGA546100.028.2135059
CTAGCAG569400.027.1388178
TCTATCG632650.024.02265716
GATCTAT647700.023.57769814
CTATCGA648450.023.50749817
AGATCTA659450.023.17343113
ATCTATC681700.022.40191515
TATCGAT685200.022.2108218
AGCAGAT718500.021.68752110
CAGATCT734400.020.96572312
ATCGATT781500.019.48275419
GCAGATC816100.018.99104511
TCGATTC807200.018.90141320
Produced by FastQC (version 0.11.5)