FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27962279
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT2092980.748501221949756No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1586350.5673178498791175No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG992710.3550175577605817No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA770440.27552832871741245No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA641450.22939832622369585No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC608250.2175251881293367No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC434310.1553199580048536No Hit
CGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACG388070.13878339458668587No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG368330.1317238841655217No Hit
TCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACC330070.11804116538569691No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC312930.11191147903216328No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG310240.11094946874680708No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG301290.10774872820630964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298180.10663651557156696No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG297610.10643266952597105No Hit
GCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATGTACCCCG293910.10510945835280451No Hit
CCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGT285720.10218051253976831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC308250.038.547341
GACTCTA475750.032.1854864
ACTCTAG483550.031.6737215
CTGACTC482950.031.632562
GCTGACT490900.031.1420571
TGACTCT498250.030.8302863
CTCTAGC542650.028.2696916
TAGCAGA544450.028.2668849
CTAGCAG569000.027.1148038
TCTATCG632350.024.02299716
GATCTAT647100.023.5779414
CTATCGA648150.023.5129917
AGATCTA658700.023.17857713
ATCTATC680800.022.41097615
TATCGAT684650.022.22356418
AGCAGAT717750.021.6663110
CAGATCT734750.020.9462212
ATCGATT781000.019.4952419
GCAGATC817400.018.94372611
TCGATTC808350.018.88319220
Produced by FastQC (version 0.11.5)