FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37387115
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1788700.4784268590930324No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1329960.3557268326266951No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG933960.24980798866133425No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA687990.18401794308012268No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA585660.15664755090089194No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC471580.12613436474036577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC317900.031.299741
GACTCTA423850.031.1225194
ACTCTAG437000.030.2825875
CTGACTC448250.029.3106522
TGACTCT467450.028.369293
GCTGACT468850.028.246951
CTCTAGC492500.026.7923626
TAGCAGA517150.025.8877839
CTAGCAG534450.024.9974758
TCTATCG560650.023.2242716
CTATCGA574950.022.70748917
GATCTAT593250.022.08948314
TATCGAT606100.021.51155518
AGATCTA617400.021.24808913
ATCTATC635700.020.65849515
CAGATCT711550.018.68266312
ATCGATT706350.018.5228619
AGCAGAT732200.018.46623610
TCGATTC731550.017.90381220
CGATTCT731400.017.87405621
Produced by FastQC (version 0.11.5)