FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37387115
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1788700.4784268590930324No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1329960.3557268326266951No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG933960.24980798866133425No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA687990.18401794308012268No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA585660.15664755090089194No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC471580.12613436474036577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA424250.031.2746934
AGCTGAC317900.031.0244031
ACTCTAG437450.030.4034375
CTGACTC447950.029.4630852
TGACTCT466900.028.560113
GCTGACT468600.028.396371
CTCTAGC492900.026.898426
TAGCAGA517300.025.9750219
CTAGCAG535100.025.0717898
TCTATCG559450.023.40552116
CTATCGA573850.022.87305517
GATCTAT592900.022.21473714
TATCGAT604000.021.67911318
AGATCTA615400.021.41387713
ATCTATC634950.020.7876815
CAGATCT709800.018.817512
ATCGATT706050.018.6219
AGCAGAT730950.018.57444610
TCGATTC732200.017.95007520
CGATTCT732400.017.9022621
Produced by FastQC (version 0.11.5)