FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20880415
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1124240.5384184174500363No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA911810.43668193376424747No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG557960.2672169111581355No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA466670.22349651575411694No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC371150.17775029854531146No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA359200.17202723221736732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330950.1584978076345705No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC253050.12119012002395546No Hit
CGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACG226890.1086616334014434No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG223780.10717219940312489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC202100.033.3884121
GACTCTA278350.030.2778844
ACTCTAG282750.029.7329445
CTGACTC285650.029.3321532
TGACTCT297100.028.4021823
GCTGACT296750.028.3411921
CTCTAGC318550.026.414046
TAGCAGA324400.026.0786559
CTAGCAG340500.025.1332728
TCTATCG371050.022.4697216
CTATCGA379500.021.96039217
GATCTAT382750.021.91982314
AGATCTA394600.021.41245513
TATCGAT398050.020.95452918
ATCTATC400500.020.90484615
AGCAGAT452050.019.06303410
CAGATCT454050.018.78634312
CACGAAT20700.018.0912461
ATCGATT463800.017.97638919
CGATTCT476400.017.44951221
Produced by FastQC (version 0.11.5)