FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-6435-CHX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20880415
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1124240.5384184174500363No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA911810.43668193376424747No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG557960.2672169111581355No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA466670.22349651575411694No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC371150.17775029854531146No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA359200.17202723221736732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330950.1584978076345705No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC253050.12119012002395546No Hit
CGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACG226890.1086616334014434No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG223780.10717219940312489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC201950.033.4652331
GACTCTA278900.030.3938854
ACTCTAG283550.029.8341645
CTGACTC286000.029.4063452
TGACTCT297750.028.5282753
GCTGACT297350.028.4488121
CTCTAGC317700.026.5948336
TAGCAGA324550.026.1959789
CTAGCAG341150.025.1982048
TCTATCG371600.022.5400416
CTATCGA379500.022.06181517
GATCTAT382900.022.01175314
AGATCTA395050.021.48548913
TATCGAT398400.021.02399318
ATCTATC400500.021.0096915
AGCAGAT453200.019.09958610
CAGATCT453550.018.89191412
CACGAAT20700.018.260341
ATCGATT464600.018.02078819
CGATTCT477000.017.52301421
Produced by FastQC (version 0.11.5)