FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27419429
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1189870.43395141452435054No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1077690.3930388192985346No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG564600.20591238424403366No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA538920.1965467625164623No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA455400.16608660960809943No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC376920.13746456937524118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340820.12429872263204314No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC282740.10311666227622755No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG274390.10007137639518314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC254550.031.4306681
GACTCTA315100.027.8351924
ACTCTAG323200.027.203015
CTGACTC325150.026.8985582
GCTGACT342100.025.5660511
TGACTCT347650.025.3496483
CTCTAGC366400.023.8814756
TAGCAGA381000.023.0219559
CTAGCAG399450.022.3001798
TCTATCG434250.019.95755216
CTATCGA448350.019.41568217
GATCTAT450800.019.3333514
AGATCTA460000.018.9695813
TATCGAT473600.018.29920218
ATCTATC482400.018.03801215
CAGATCT540800.016.36842712
AGCAGAT547900.016.32223510
ATCGATT545000.015.95321919
CGATTCT567650.015.27361421
TCGATTC576050.015.06892920
Produced by FastQC (version 0.11.5)