FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27419429
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1189870.43395141452435054No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1077690.3930388192985346No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG564600.20591238424403366No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA538920.1965467625164623No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA455400.16608660960809943No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC376920.13746456937524118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340820.12429872263204314No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC282740.10311666227622755No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG274390.10007137639518314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC253950.031.3257581
GACTCTA314400.027.9750844
ACTCTAG322500.027.3380225
CTGACTC325500.026.912642
GCTGACT342200.025.5994871
TGACTCT347850.025.4155583
CTCTAGC365750.023.9621476
TAGCAGA380050.023.0978769
CTAGCAG399000.022.3428278
TCTATCG433000.020.03132816
CTATCGA447150.019.49909417
GATCTAT449850.019.40529814
AGATCTA458650.019.04830613
TATCGAT472050.018.38894818
ATCTATC480400.018.13496215
CAGATCT540150.016.41401312
AGCAGAT547300.016.35928310
ATCGATT543900.015.99835219
CGATTCT566250.015.32373521
TCGATTC575100.015.11816320
Produced by FastQC (version 0.11.5)