FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21952154
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1191640.5428351131283062No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA1174540.5350454447431445No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA568550.25899508540255317No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG545080.2483036516598781No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA466140.2123436269625295No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC404140.18410038486428257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300880.13706172068581518No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG298320.1358955481088553No Hit
CGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACG293910.13388663363057676No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC274520.12505378743243148No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC235640.10734254141985337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC249450.034.7539021
GACTCTA320200.027.1625844
CTGACTC326300.026.5469282
ACTCTAG330850.026.3308415
GCTGACT334350.025.8347871
TGACTCT343500.025.3606993
CTCTAGC368150.023.5400146
TAGCAGA380800.023.061719
CTAGCAG402000.022.0195588
TCTATCG451550.019.09225516
GATCTAT455850.019.019414
AGATCTA458000.018.96068613
CTATCGA464350.018.60348917
ATCTATC489550.017.7532315
CACGAAT26250.017.5997181
TATCGAT494100.017.51158118
AGCAGAT523450.017.06460610
AATCTAT47800.016.9139561
CAGATCT526550.016.72498512
CGAATGT29300.016.6038281
Produced by FastQC (version 0.11.5)