FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25030327
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1048260.4187959669883658No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA972170.38839684355701787No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG524640.20960173632569803No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA488430.19513528528812268No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA442040.17660176792736265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338670.13530386558673405No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC306350.12239152928365658No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG271280.10838052575182099No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC259080.10350643840969397No Hit
CGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACG253530.1012891281843821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC227600.032.046041
GACTCTA281750.027.6520614
ACTCTAG291450.026.852165
CTGACTC297350.026.3299772
TGACTCT312000.025.0604953
GCTGACT313300.024.9446431
CTCTAGC329250.023.8124076
TAGCAGA346000.022.8523889
CTAGCAG362850.022.0804758
TCTATCG393300.019.75764716
CTATCGA405450.019.20007117
GATCTAT420750.018.6679714
AGATCTA434900.018.09281313
TATCGAT432100.018.01582318
ATCTATC440900.017.78319515
AGCAGAT503250.016.06659910
CAGATCT504450.015.73020612
ATCGATT502650.015.56382519
CGATTCT520300.014.99543321
TCGATTC522150.014.94894620
Produced by FastQC (version 0.11.5)