FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0830-183-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25030327
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1048260.4187959669883658No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA972170.38839684355701787No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG524640.20960173632569803No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA488430.19513528528812268No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA442040.17660176792736265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338670.13530386558673405No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC306350.12239152928365658No Hit
TATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACG271280.10838052575182099No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC259080.10350643840969397No Hit
CGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACG253530.1012891281843821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAC228300.031.993731
GACTCTA282100.027.5184654
ACTCTAG292100.026.6965145
CTGACTC297350.026.2357792
TGACTCT312050.025.0003683
GCTGACT313150.024.8559721
CTCTAGC329500.023.7199526
TAGCAGA346350.022.7888879
CTAGCAG363400.021.998918
TCTATCG394450.019.63794316
CTATCGA406250.019.0847317
GATCTAT421050.018.61311714
AGATCTA435150.018.04221513
TATCGAT432500.017.91824218
ATCTATC442750.017.66146715
AGCAGAT504100.016.01869210
CAGATCT505050.015.67687712
ATCGATT503150.015.46488819
CGATTCT520450.014.93731721
TCGATTC522400.014.88149920
Produced by FastQC (version 0.11.5)