FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC33.75Illumina Single End PCR Primer 1 (100% over 50bp)
TCCGGCGTGCAGAAGCTTCTCCGGCGCCAACCCGGCCCTTCGGGTGCTCC11.25No Hit
GCAGGAGCTGCCTGGAGGCGGGCTCCTCGTGTACCAGAGCTTCTGTGCTG11.25No Hit
CTCAAGATCAAGGCCGTCGTCGCTCACCGCTGCGTGCGCTCTCAAGACGC11.25No Hit
GTGGGCCTGAAAAACCAAGGAGCTACCTCTTATATGAACTCCCTTCTACA11.25No Hit
CTTCTACGTGGTGGGCCGAGGCTTCGGTAACTTCGACAAGGACAAGGACC11.25No Hit
TTAACAAAATCAAAACGCTGGACAGTGGCCAAGCGCATCAATGTGCAAAC11.25No Hit
AAAGGCTCCAGAGTTTAAGTTTGGCTGGAGGACCGACGAAGAGTTTGCGA11.25No Hit
GTCTCATAGGAGAGCTGCTGCAGCCACGGCCTCTGGAAAATTCAAGGATG11.25No Hit
AATACACTGGGCTTCTACAAGCAACCAAAGATATTCTCAGAGAGGAAGGT11.25No Hit
GCGGAGGCTGCCAGAGAGGACGCCGGTGCAAATAAACGGCACGGCGGCAC11.25No Hit
GAACAGATTGATGTCGTCCTTGATGACAAGGGCTAATTGAAGATGTTGCA11.25No Hit
CGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCT11.25No Hit
CTACACTGTTCTACCCATGGCTTGATAGTAAATGGATGCCTCACCCTCTA11.25No Hit
CTTCTTTCCCTTCCACACAACACACAGCTCACTCAACTCCCCTCCCGCTT11.25No Hit
GGTAGTGCCAATTGGCCGAACCTATTCCAGCCAAAGGCATAAATTTCACC11.25No Hit
AAAAGACACAGCTAGGATACCGAACTTATGGGTCGTTCAATTTAACAGTT11.25No Hit
CTTAAGTCGCGTGGAGTATTCACCGAAGCAAAGTGGTACTGGATTGGCTT11.25No Hit
GAACAGTCAGTTCTCCTAACCACTGAGCCATCTCCCCAGCCCCCGAGTTC11.25No Hit
CCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG11.25Illumina Single End PCR Primer 1 (100% over 48bp)
CTCATGCGAACTTGCTCAGACTTGTGGATTTGCTACAGGAAGGATGATGT11.25No Hit
TGGGGGTTCTACACCGGGATCATGGAGGACGCGCGGCCGGCCGAGGGTGC11.25No Hit
TGCACTCCTCGCCCTACGGGTTCGAGTCCAAGGCGGTCCCGGCGGCGTGG11.25No Hit
TTCCCCTAATTTCCCCATTGGAAAAAGAATCTCATCTGCAAAAACGATGG11.25No Hit
CTCGTCAATGCCTGGTTCCTCGCCAACGACCCCAAGAGGTGGGTGCGCCC11.25No Hit
CATTGTTGAACAAGTTCGTGATGGCAGTACTGTTCGTGTGTATTTACTCC11.25No Hit
AAGCGTTTAACACCGAACCACTATGTGAAAATCTTGACAGATTTCATGTC11.25No Hit
TTTTTTTTTTTTTTTTTTTTTAGCGCTCGAATGTAACCGTTTAAATGTGG11.25No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTGGGGGGGGTGGGG11.25No Hit
TTTTTTTTTTTTTTTAAAATGAACCAAAAATTAGAATACCTGCGATTAAT11.25No Hit
TTTCAGGCAACCGGTTCACTGGATCGGTGCCGAGAGCACTTGGCAGCCTG11.25No Hit
CGGGAAGCTAGACATCCTGGTAAATAATGCCGCGATTGGCGGGGTTGAGT11.25No Hit
GTTGAACATAGTGATACCAATCCCCAGATAAAAAGGTCAAAATAACCTTT11.25No Hit
GCAGTTTGCATACTATGTACAAGCTCTTCCTTTCTGCTGTGGAGTATCTG11.25No Hit
CAACACATTAGCGGGTGCTCATGCAGGAGCACGACAGTTAGAGGTGACTA11.25No Hit
GGCAAATGGTTCCGTATGTACGGTGCGTGGTGTTTAGTTTTTGCAAGTAG11.25No Hit
CGCCGGCCGCCGCCTCAACGCCTCTTTCGTCGAGCTCTTCATCCGCGAGT11.25No Hit
CGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCG11.25No Hit
TTTTTTTTTTTTTTTTAAAGGAAAAGTTTTTTAAATAATTAGGAAACAAA11.25No Hit
GGCACTTGCTGAGAAGCACCTGGCTGCCCGCTTCAACAAGCCTGACAGTG11.25No Hit
ATTCTCTGTCGCGGTCTCCCCTGTCTACCTTCTTTGGTATCATGGCGCCG11.25No Hit
GCCCGCTCACCGACGATGAGATTATCCTCCTATGCTCCGAGTTCCTCGAC11.25No Hit
TTTTTTGCGCATAGAAGAAATCCAACCCGTCCTTAGCTTCACTAATGGAA11.25No Hit
TTGATAGTGACACATTACAGCAGCTTGATTCATTTAGAGGAATGCTGGGA11.25No Hit
CCAGGACTTCTGCATCGCCGACCTATCCGCCGCCGCCACGCCGGGCGGCT11.25No Hit
ATCATTTTTTTCACCTCTATGGATTCTGTTATGTCTTGAAAGATGACCAG11.25No Hit
CTGCTCGAGTCCCCTGATGCTCTCAAGTCCAAGGTTGCTGAGGCGATGGA11.25No Hit
AAAGATTGCAAGCTTGCTGATATTGTCATTAGGGGTTTACTGAAGTATTG11.25No Hit
CTCACAAACTTCTGCCAGAGCAGCTGCCCGGAGGCGCCGGCGCCATAGGC11.25No Hit
CGGCGTGGTCTGACCATCGACCCGGCCGGGGAGGAGGAGGCCCCCGCGGC11.25No Hit
CGCAATGGCAGCTCCTCTTCTCCTCATCGTCGTCCTCGTCGCCACCACCG11.25No Hit
TTTTTGACATCACTGTAAATGGCGTCCCAGAGGCGGGAAATTACCACGCC11.25No Hit
GGTTGAAAGACTGTGGTGATCTAGAGAGCTGATGGGTGACTGGTGCAAAA11.25No Hit
TAGATAGCCTCTGCATGCCTACGTACAGCATGGCCAAGGCCGAGCTGCTC11.25No Hit
CCAGCTTGCTCTGGTTGAACCTGGCCGACAACAACCTGACCGGGAACATC11.25No Hit
TGGGAGCACCTGGGAGGACATGATGCGGAGGATCCTTCCCCCGGGCACTC11.25No Hit
TTTTTTTTTTTTTTTTTATATTAAAGTGGGTTTTTGGTCCAACAACCTAA11.25No Hit
GTCAACAGATCGGAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG11.25Illumina Single End PCR Primer 1 (100% over 30bp)
GGTAAAGAAGACTAGTTATGAGAGAGATTGTTTGTAGGAATCATTACCTG11.25No Hit
TTTTATTTTTTTTTTAATAAAAGGCAAGAATTAAATTAAATAAAAAAACT11.25No Hit
GAATAGTTTGAGTAGTACTGGTATTAGGTCTTCCTTGAAGCCCTGATGGA11.25No Hit
CTACACTGTTCTACCCATGGCTTGATAGTAAATGGATGCCTCAGCCTCTA11.25No Hit
GTGGAACTACGTGCCCCTGCTGCGGTGGCTCGATCTCTACGGCGCCAAGA11.25No Hit
GGGTCTGACAAAATTACCTCCTGGTCCATCGTGTGGTTTGCTTTCACCAC11.25No Hit
TTTTTTTTTTTTTTTTTAAGGGGCCGTGGCAGCCCGCCGGGCGCCCACCC11.25No Hit
CGCAGCTATTTTAAATCCGGGTAATGGCTCGTTCATAGTGGCTTAATCTT11.25No Hit
CGCCGGCGTTCGGCTCTGCGTGACTCTGCCCCAGCCCAGAGCTCTCCCTG11.25No Hit
TCAGTGGCAGGCCCAAGCTTGAGGGACAACCCAAAGTTCTTCATCCAGAT11.25No Hit
AACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG11.25Illumina Single End PCR Primer 1 (100% over 44bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC11.25Illumina Single End PCR Primer 1 (98% over 50bp)
GGCGACGGCACCAGCAGCAGCAGCAGGAGCAGGTAATGGCTATGGCACCG11.25No Hit
GGCGATCCCGAGAAGTTCAAGGAGCTAGCTCAGGCTTATGAGGTCCTCAG11.25No Hit
ACAAGTCATCAGTTACTAGCTAGTTAGTAGCCTATACAACACATACATAC11.25No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGCGCGTGGGGGG11.25No Hit
AGATCTTTGGGGCTGGAGTCATTTGGGTGAGATCGGAAGAGCGTCGTGGA11.25Illumina Single End PCR Primer 1 (95% over 21bp)
ACAGACTCAAGATTACGACCAGACCAGAGATGTCTGGAGAACGGTGAATA11.25No Hit
CCCCCGTGTCGCATCGCATGGCGCCGACCGCCGAGGGCCGAGGCAGCGCC11.25No Hit
CGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGA11.25No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGA50.070.044
TTTGAGT50.070.07
CTCTGCA50.070.052
GATGGAA50.070.045
TGCACTA50.070.055
TGGTCCT50.070.070
TCCTTGA50.070.032
AAACCCT50.070.061
CCTACTG50.070.065
ACTAAAC50.070.058
GTTTGAG50.070.06
TAAACCC50.070.060
TGGTATT50.070.019
AGTAGTA50.070.011
ATTAGGT50.070.023
GGTCTTC50.070.027
GTACTGG50.070.015
GAAGCCC50.070.037
CCTGATG50.070.042
GTATTAG50.070.021