FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26621778
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT596050.223895639126733No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA553230.2078110635585647No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG329990.12395490639280367No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA302440.11360623621758095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275980.10366700526163203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG190900.024.4213335
GACTCTA195900.023.9765034
AGCTGAC190800.023.8097211
CTGACTC207800.022.0471322
TGACTCT217450.021.5518653
CTCTAGC224000.020.8754586
TAGCAGA244150.019.4537779
GCTGACT245550.018.9284971
CTAGCAG257100.018.6236178
TCTATCG248400.018.21944416
CTATCGA260650.017.37656417
TATCGAT277300.016.39632818
GATCTAT292200.015.82377114
AGATCTA310300.014.810492513
ATCTATC318700.014.47509815
ATCGATT335500.013.68761719
CGATTCT339100.013.47007721
TCTAGCA546050.013.22989757
TCGATTC348450.013.16882820
TTCTAGC360050.012.66676524
Produced by FastQC (version 0.11.5)