FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27265418
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT572500.2099729408146246No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA559540.20521966690552848No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA286430.1050524881004942No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG276490.10140684437700534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA194550.023.3689774
ACTCTAG194300.023.14725
AGCTGAC196000.022.7312721
CTGACTC202300.022.09272
TGACTCT218050.020.657773
CTCTAGC228850.019.4541176
GCTGACT238400.018.8499491
TAGCAGA240300.018.643959
CTAGCAG257200.017.8951979
TCTATCG247000.017.68505917
CTATCGA257550.017.04213718
TATCGAT274900.015.87741318
GATCTAT280800.015.655848514
AGATCTA298600.014.89840614
ATCTATC311600.014.19826515
ATCGATT324800.013.48119920
CACGAAT33550.013.2483751
CGATTCT332200.013.14933521
TCGATTC346000.012.81704120
TTCTAGC351650.012.49164324
Produced by FastQC (version 0.11.5)