FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26746497
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT536260.20049728381253068No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA504890.18876864510518893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA178650.022.3340514
ACTCTAG177800.021.9689586
AGCTGAC184600.021.6324541
CTGACTC188650.020.779033
TGACTCT203850.019.658883
CTCTAGC210050.018.6628177
GCTGACT220250.017.9722562
TAGCAGA226400.017.6555210
TCTATCG223000.017.12431317
CTAGCAG248550.016.6594319
CTATCGA235000.016.45837218
TATCGAT246800.015.52961119
GATCTAT256750.014.96868115
CACGAAT35450.014.6115851
AGATCTA279350.013.87050213
ATCTATC284250.013.58209115
CGATTCT296900.012.81484721
ATCGATT303850.012.67139120
TCGATTC319200.011.99633321
TCTAGCA486800.011.9857987
Produced by FastQC (version 0.11.5)