FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22.247191011235955No Hit
TTTCCCCAAAAGCTACTTGTGTGTCAGTTATGTGGTCAGCAGTGACCACT11.1235955056179776No Hit
GAAGAACACAAGCTGTCGGATGAGCTTCGCGACAAGTGCAAGGAGCGCGG11.1235955056179776No Hit
CGACGATGACGATAAGTATCCGATGGCCTCCTCCGAGGACGTCATCAAGG11.1235955056179776No Hit
TTAAAATGGCTTCTGCCACAGCCTGGGATGCAAGAGGCTAGGATTTTGGC11.1235955056179776No Hit
CTTGCATTGGGTGGTGGCCTAGAGTTGACTATGGGTTGTCATGCTCGGAT11.1235955056179776No Hit
TGTTTATTAGTTAATATATGATATTTATCAAAAATAGGAATAATAATATT11.1235955056179776No Hit
GCCATCGTTTCCAAGTACGCGCAGAGAGAGAGAGCCACAAGAGTTAGAGC11.1235955056179776No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGATCTCGGTGGTCGCC11.1235955056179776Illumina Single End PCR Primer 1 (98% over 50bp)
CACTGGCCTGCGCGGAGAGCAGGCGGCACACACTTTCCAGCAACGGCCGC11.1235955056179776No Hit
CTCAAGGATGCTGTGGAAGGAGGCTGACGGCAAGCCTGACTGGGTGAAGG11.1235955056179776No Hit
CTTCCATACCGTAGTTAACTATCTTCACATTGTTTTCTGTTTTTTTTGGG11.1235955056179776No Hit
TTTTTCTCAGCCAGATTTTGCTGAGCATGTTGACTTTCTAGAAGCCATAA11.1235955056179776No Hit
AAGAAGATGTTGTGAGCTGTGCTCTTAAACTCTATCTCTATCTACTCTCA11.1235955056179776No Hit
TTTTTGTGTTCTTTGGTTATCTATGAACCATTATCCATGTAAAATAATAC11.1235955056179776No Hit
GGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGA11.1235955056179776No Hit
CCTGAGTGAGATTGTAAATGGTCCTTATCATTGTTGTCTTCCTCGTTGTG11.1235955056179776No Hit
CGACCTCATCGTCCCCAGCCCCGAGTTCTCTGCCAGCGCCCTCGTCTCTT11.1235955056179776No Hit
GGAAGAGGAGACAAAAATCGGAGAAATCATGCGATTCGATGAGGATAAGT11.1235955056179776No Hit
CCTTGCTTTTAGGGACGAGCAGTCCTGTAAGGTTGCAGAGAGTATGGTTA11.1235955056179776No Hit
GGGCGTGACAGGACTTCCATGCATGTGGTGAAGCCCAAGAGGAGGAGGTT11.1235955056179776No Hit
ACACAAAGTGCAGCAGATCAGGGAATTCACCTGACTACAGACAACAGAGC11.1235955056179776No Hit
TGCTAGATAGCCTCTGCATGCCTACGTACAGCATGGCCAAGGCCGAGCTG11.1235955056179776No Hit
GGCGGCCACTACGACGCCGAGGTCAAGACCACCTACATGGCCAAGAAGCC11.1235955056179776No Hit
TGCCATCGCACGCGGATCTGGACCGCCAGATCTCGCAGCTGCGGGATTGC11.1235955056179776No Hit
GTTGCCCCGCCGCGCAGAGGAAGAAGAGAGCAGAGGAGGATGGCTCGTAC11.1235955056179776No Hit
AGGGGGTACAGATTTAGTTTGTCAATCTGGACCTGGATCATGGCCTTCTC11.1235955056179776No Hit
AGCCACGAGCTGGAGTGGCAGCAGCACGCCACGCAGACCTCCACCGCCGG11.1235955056179776No Hit
ATTCCTGATCCAGATGCAGTAAAGCCTGATGACTGGGATGAGGATGCCCC11.1235955056179776No Hit
CTACCACGGATGGAGGATTAGCCACAGGACGCACCATCAAAACCACGGCC11.1235955056179776No Hit
GGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGG11.1235955056179776No Hit
ATGCTGTCATCGCGTTAGCCGTGCACATCCGCGCGCGTAGCGTAGGTCGG11.1235955056179776No Hit
GCCAAGAAGAAGGCAAGCCTGATCGTCAAGATGTGCCAGAGAAGCCAAAT11.1235955056179776No Hit
TTGTCTGCCTGTTTACTTCAATGAGCCACTTTCATCGTTACAAAAATGTT11.1235955056179776No Hit
ACCTGCTCAAGTTCTTCATCCGCTTCGTGCTCAGCGGCAGGGCATGGGAC11.1235955056179776No Hit
CTGGGCTGTTCTGGGCACTGCTGCTTGCGGTAACTGTTGTTGGGATGATT11.1235955056179776No Hit
GCTCGATGCCAAGCTGCAGGGTTTGCACCTGAGCACGAACCTGTACGAAT11.1235955056179776No Hit
AGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTC11.1235955056179776No Hit
CAGTTCTACTCACTGATCACCATGTGGCTTCTAACTACAGCATGCGGAAT11.1235955056179776No Hit
GGAGAACCCCGACTACCACCACCCGAAGCTCAACACCAAGTTGGGGGTCT11.1235955056179776No Hit
TCCTTGGACCTTTTTTTTCTAATGAAAGTCTTTTTAGCCTTAGTTGAATT11.1235955056179776No Hit
ACAAGGCGTCCAAGGCGCTCGCGAACGCCTATTCCCGGATCCTTGCGAAG11.1235955056179776No Hit
CTGTGCAGGTGACGGCGGCGCGCTACCGGTGACAGGGGATACGGCGGCAT11.1235955056179776No Hit
CTAAAAGCATCTTTGGACCCTGAACCATGTTGTATCTGCCAGGAGGAGTA11.1235955056179776No Hit
CATAACTGATATATTACAAACTAAACTTATACATAATCTGGCTTCTCTTC11.1235955056179776No Hit
CAATATTCGATCAGTTCCAGAGAGAAAGGAACGCTGATCATTTGAAAACC11.1235955056179776No Hit
TGGCTCCCTGCGTTGCGTCTTGGGGGAGAGAGAGAAGTGGGAGACCCGCG11.1235955056179776No Hit
GCGCTAGAGAACTGTGCAGGGGGGAGTTCCTGGCCAAGCATGCCCATTAT11.1235955056179776No Hit
CGACGACTACATGTACCTGGTGGCCAAGGAGAACAAGTGCACCCTTCCTT11.1235955056179776No Hit
AACAGTGATCGTGATGCACTTGAGCAATCAGTAAAAAGCGCTATGGACGT11.1235955056179776No Hit
GGGACAGTGCACCAAGCTCACACGCCTGAAGATGGATGGCAACAGCATAT11.1235955056179776No Hit
GGCTGATCGTATCATTGGAATGCGGAAGGCACTTAAGGAAAATCTTGAAA11.1235955056179776No Hit
GCGGCGTACCAGGTGTCCAAGGCGCTCGTCTCCGCCTAAGCCAGGGTCCT11.1235955056179776No Hit
GGGGGGGGGGGGGGGGGGGGGGTGTGGCCGGGGGGGGGGGGGGGGGGGGG11.1235955056179776No Hit
CGAGATCTCCGTAAGAAGGCGGAACCTGTAGAAGACATGGCCGGCGAGAT11.1235955056179776No Hit
ATGCTCTGTCTCATCTACACCACCTCGGCACAAGCACGAAGGAGTGGCTA11.1235955056179776No Hit
AACAAGTGCTCTACACTATTTCTAGTTGCCGGTGCGATGACGATAGGCAA11.1235955056179776No Hit
GGAAATCCTCCCATCTCTGCCTGAAGAGAGCTGGGATTAAAGACATGCGC11.1235955056179776No Hit
AATGTCGTCTATGGGGATCTCACACAAGTTTTGAGTATTTTGTTCAACTG11.1235955056179776No Hit
CGTCGGGTTCTTCGCGCCTCCGGCCGCCGCGCCGGCGGCCAGGATCAAGT11.1235955056179776No Hit
TTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTT11.1235955056179776No Hit
GTTGCTGACAAGATTGTGTACGGCGGCAAGGCTGATGAGAAGCAACTAAA11.1235955056179776No Hit
GAGTACCACTACCGACATGATGCATGCCTAAGCTTTTGCTGTTGTCGTCT11.1235955056179776No Hit
ATTTGCTTGCTGCTTCGATGTTCATATGACATGCACGTACTGTAGCGCAA11.1235955056179776No Hit
GGTAGTGGCGTTGGTAACGACTTCGGCGACCAGAGGCAACGTTACGGCGG11.1235955056179776No Hit
GTATCGCCGGCCGAAGGGACAAGCCGCCCATTGCACACCAAAGGCGGACC11.1235955056179776No Hit
GAGGAGGGCACTGACGACGGCTCAGCCCCTGGCCGGTGGGTCGTGTACAG11.1235955056179776No Hit
TTGTTTTTCTTGAAGAAACATCTACATATGGTTTCTTCAGCTCTCACTCT11.1235955056179776No Hit
CTGTCGCTCCAGCCTATCGCTGCCTGTCCGCACGCCCTGGGTATCTCAGC11.1235955056179776No Hit
TTGCATATCTTGGTCTTAATTTTGCCATTCTGATTGCCGATGTGGCTGGC11.1235955056179776No Hit
GCGCCGCAACTCATCCGGCGGCGGTGCGGCGGAGGAGCTGCAGGCCGGCA11.1235955056179776No Hit
TCAAGCACTGTTGCTGTGATACAAGGGGATGGTGGTATCTCTGAAAGTGC11.1235955056179776No Hit
GAAGGATATTACAGTAATATTCAGAAGGAGGGGTGGTTGTGATCTTGTTC11.1235955056179776No Hit
AGGAAACACTACTTAGGCTTTTAAGATTGCGTTGCGGTTTAAGTTCTTAT11.1235955056179776No Hit
AAGGGACCGAGCTGAGGGAGGTGTCCGAGGCGGTGGCAGACCTCTTCTAG11.1235955056179776No Hit
TCAAAAAGGACTGCGACCCCGTCTGCAAGAAGTGCGTCGTCGCCGTGCAC11.1235955056179776No Hit
TGCGGTGGCAAAGGCTGAGATTGACCCCGACGACTACTTCAGGAATGAGC11.1235955056179776No Hit
GACGATTTCAGGAGATGCTGGAGGTGACTCTGGGACAATGTCGGGGCAGC11.1235955056179776No Hit
AGATACATAAATGTATTACCTCAATGAATGCCATAGGATGAGATATTGGT11.1235955056179776No Hit
GGAGCTCAAGCGGTGCCTGGTGGACACGGTGTACGGCTCCGACCTCGGCT11.1235955056179776No Hit
CAGCGACTCATGTCTGCCTTGGCTTGACAAGCAGCGTCCGCGTTCTGTAG11.1235955056179776No Hit
ATGAAAATGACACCCAAGCTCTTTTCAACTCTCTGGAATCTTGTTGTTCA11.1235955056179776No Hit
TCTCCGGCTCTGCGTCTGCGGGCGCTGACCTGCCAGTTGCGAGGAAGGCG11.1235955056179776No Hit
GGTAACGGAAACCCTAATCCCCCCAAATCCCGAATCCCTCTCGAATTTCT11.1235955056179776No Hit
GGGTGGTGCCGGGGAAGCTCAACGTGCACGTCGTGCCGCACACCCACGAC11.1235955056179776No Hit
TGTGCCACTACTTTCCCTTCCTGCATCAATACACATCCTAGTCCGATGTA11.1235955056179776No Hit
AATCTATCCAAGCAACAAACTTGGCATTCTTGCCTGTTCTTAATTATTGC11.1235955056179776No Hit
TTCGACGGCTGGCGCCTCGACTTCGCCAAGGGCTACTCGCCGGCCGTCGC11.1235955056179776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTCA50.070.061
GATGGCT50.070.039
GGATGGC50.070.038
TCAAGTC50.070.065
GAGGATG50.070.036
CGCCCTC50.070.060
GAGGAGG50.070.033
GAGGAAG50.070.017
AGAAGAG50.070.022
AGGATGG50.070.037
CCGCCGC50.070.07
CCGCCCT50.070.059
GAAGCAG50.070.051
ATGGCTC50.070.040
AGAGGAG50.070.032
AGAGGAA50.070.016
AAGAGAG50.070.024
ACCGCCC50.070.058
AGGAAGA50.070.018
CGAAGCA50.070.050