FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23046101
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT576970.250354712929532No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA523900.2273269565207581No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274480.11910040661541837No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG267890.11624092075271214No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA259260.11249625261991172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG175800.024.1898355
GACTCTA182150.023.7689174
CTGACTC182100.023.409752
AGCTGAC179250.021.8879431
TGACTCT195700.021.8370383
CTCTAGC200650.021.4035856
TAGCAGA209550.020.5784039
GCTGACT212150.020.0938321
CTAGCAG226750.019.3415588
TCTATCG221950.018.92424416
CTATCGA234500.017.95623217
TATCGAT247800.017.02073118
GATCTAT249400.016.967614
CACGAAT25050.016.626381
AGATCTA264250.016.08019813
ATCTATC270850.015.63673715
ATCGATT287950.014.74473919
CGATTCT296750.014.16585621
TCGATTC311250.013.59594420
AGCAGAT336600.013.28948810
Produced by FastQC (version 0.11.5)