FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25752332
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT655370.2544895739927553No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA587430.22810749721617446No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG333970.12968534267110257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292620.11362854439745496No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA291930.11336060749760447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG196450.025.1209665
GACTCTA203700.024.6045464
AGCTGAC187200.023.7065941
CTGACTC209350.023.4717622
TGACTCT224050.022.1822243
CTCTAGC230500.021.4710676
GCTGACT244300.020.3432661
TAGCAGA244850.020.1130359
TCTATCG250850.019.2557116
CTAGCAG267300.019.026128
CTATCGA261400.018.54554417
TATCGAT277850.017.46005818
GATCTAT283000.017.26587114
AGATCTA304750.016.11406713
ATCTATC307200.015.81461415
ATCGATT332250.014.6750319
CACGAAT34850.014.6624251
CGATTCT340050.014.27660221
TCGATTC350650.013.94476520
TTCTAGC358950.013.49537324
Produced by FastQC (version 0.11.5)