FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGGCGTGTACACCAAGAAGAAGCCGGCCAACCAACCGACTCCCCCGA23.7037037037037033No Hit
ATCGATTCTAGCATGACTGGTGGACAGCATGGGTCGGGATCTGTACGACG11.8518518518518516No Hit
TCCATATTCGCCACATCTTCTTACGTAGCTCTCTCTCTCTCTCGCTACAA11.8518518518518516No Hit
TTCAAATCAACAAGAAAGGAATCCTTCTATGATCTCCAGCTTGATGTCAA11.8518518518518516No Hit
CAGGAATTGGAGCCCAGGCTCTAGGAGAGAAACTAATACTATTTTTCTGG11.8518518518518516No Hit
GCTAAATTGAGACCGTTTAATGCTGCGGTCTGATCACCATCACAGCATTA11.8518518518518516No Hit
GCTTCTCCCCTCTCCTCGACTCCTCCTCACGTCACGCACCCCATCTCTTC11.8518518518518516No Hit
CTCCCTCTCCGAAATCAAGGTAGCGTACACCGCTGCAGTTTCTAGAGCCC11.8518518518518516No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCGGAGAT11.8518518518518516No Hit
TGAAGGTCCTGGAGTACGGCACCGTCGTGGAGGTGGTGTTACACGACACG11.8518518518518516No Hit
TTGGGCTCCAGCTCCACCAGGTATTGCTAGTCACCATAGGAAGGAACCTG11.8518518518518516No Hit
CTCCACGGGCGGGTCGCGGATACTGGACGGGTACCGGCCGGCGTACGACG11.8518518518518516No Hit
TTCATTTGTTTAATCATTTTGTTCTCACCTCCTCCTGATGCGATCAGCGC11.8518518518518516No Hit
GTGTTTCTTCCAGGTTCTATCTTTGTGCTGCTTTTGATGGTGTTAAGTGC11.8518518518518516No Hit
GGTGAAGACAGCCCTTCAGAGAAGGCTGCTGAAGCATGAAAGGTTCTGCC11.8518518518518516No Hit
GGATATTTTACTTCATTGGATTTGGAATGTTCTGCCTTGAATCACTGCTC11.8518518518518516No Hit
TGGCAGAGACCTGGCTACCCAGATACAAAGTTGTGGGACTTGCATGTTTC11.8518518518518516No Hit
GGTTTCCCAATCAAAACTACATGGTTTTGCCCAACGAGATCATAGAGGCC11.8518518518518516No Hit
TACATTCGCCGTCATAAAGATGGCAATGGACTAGGCAGCTCTAAACTACA11.8518518518518516No Hit
GGGACTTGTACGCGTTCATGGCACCGTACGTGAGCTCCAACCCGAGGGAG11.8518518518518516No Hit
TCCGGAGGTACTGCAAGGCGGAGCAGCAGGTGGTCTCGGCGCAGACGGCG11.8518518518518516No Hit
CAAACAGCAGCGAGCCAGCAAGGATCCCCACCCCCCCCGCCCAACAAATT11.8518518518518516No Hit
CACGCCCTGGGTATCTCCGCGTGGTTCCGGCAGGAGCAGCACGAGCCGTC11.8518518518518516No Hit
GAGACGGGGCGCACGGCGGGCGCGTCGGCCGTGATCCTCAACACCTTCGA11.8518518518518516No Hit
GCCCACTTAGATCACGCTCCAAGTTGTAGCACCCAAGTCGCGTTTGGTAT11.8518518518518516No Hit
CGGTACGGGCACACCGAATATCCCGTGGACCGGAGAAAAGCAATCAGATA11.8518518518518516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGATTGGGGGTTTTTTTTTGGTAATTT11.8518518518518516No Hit
GGGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGAT11.8518518518518516No Hit
GGATGTTGAAAAGCCCTTCTCGAATCTTGAATACACGGGAATTAACCGGG11.8518518518518516No Hit
GGGGGGCGACAGCGTGCGGAGGCGCCTCGGAGGAAACCTAAAGGAGGAGC11.8518518518518516No Hit
CAACGCCTACTGCGACCCGACCAAGAGGCAGTGGCCGTGCGCCGCGGGGC11.8518518518518516No Hit
CAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGC11.8518518518518516No Hit
GAAGGAGTAATTCAATTAATTAAATTTAATTAAATTTTAAACTCCTTTTT11.8518518518518516No Hit
TGCCTGCCTGGATACTGCCATGCTCTCACCTTGATGATAATGGACTGAAC11.8518518518518516No Hit
TAAAACGCGTGGCTTTTGTGTGCCTCTGATGATGGGCTTTTTTTCAGAGG11.8518518518518516No Hit
CTAAGACAAGCCTGGGCTAAACCACACTAGCGGATTTTCTTAGTGGACTC11.8518518518518516No Hit
CTCTTCCTCCTCCTCGCGAGGGAGGCGATCCAGTCGTATGAGTGAAGTAA11.8518518518518516No Hit
GGAGACTCCTCTGCATATGGCAGTGAAGAACAGTTCTTGTGGATCAACAA11.8518518518518516No Hit
CATGATCTCCCTCACCGTCTGCCAGATCGTCGACACCGCCGTGCGCGAGG11.8518518518518516No Hit
AGCTGGTACCACATACCACGCGAATGGTTGCAAGAGTCAGGCAATCTATT11.8518518518518516No Hit
CTCCCACCAGCGCTTGGAAACTGTACACGTCTAAGCACCCTTCACATCAG11.8518518518518516No Hit
ATCAACTGCTACCGCGTGCTTGACGACGACGGCAGAACGATATCAGGAAG11.8518518518518516No Hit
GCAGGTCGCGTCCTCGCTCAAGGGCGGCGTCGTCCAAAGATGGGCTCATC11.8518518518518516No Hit
TCCAAGGCTGCTTAGATGGGTCCTTCAACAGAAGGAGGTATATTCAGGGG11.8518518518518516No Hit
GTCCCCTCAGTTCAAGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCG11.8518518518518516No Hit
ATCGACCTGTGTATTGGAAACTGGACACCTCTGCTGTAAAAGAAAATGGA11.8518518518518516No Hit
AAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTA11.8518518518518516No Hit
AGCGGCTGTTGGTCATCGGTGGACCGAGAGTGCTGGGCGGCGGGATGATC11.8518518518518516No Hit
CCACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCGCCGAGGGCC11.8518518518518516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGTGGGGGGGGGGGG11.8518518518518516No Hit
CCACTGCCAGAAGCCATGGAGCGTCAAGGAAGACTGATGATAACGCCTGT11.8518518518518516No Hit
TCTATGTGCGTCTCTCCTATCTCACTGCGTCCCTTCCTGAACCCTGTGTC11.8518518518518516No Hit
CCTCTCTCTTATCTTCAACTTCCTCAGTCTTCTCTGAAGCATCTACACAA11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG50.070.065
GTATGGC50.070.054
TGGTTCC50.070.022
ACGCCCT50.070.02
ATCTCCG50.070.013
CAGCACG50.070.037
CGCCCTG50.070.03
CTGGGTA50.070.07
GGCGTTC50.070.063
GGGTATC50.070.09
TTCCGGC50.070.025
TTCCGCC50.070.067
CACGCCC50.070.01
GTTCCGG50.070.024
GTTCCGC50.070.066
GCAGGAG50.070.030
CCGCCAG50.070.069
GTATCTC50.070.011
ATGGCCT50.070.056
CGCCAGA50.070.070
Produced by FastQC (version 0.11.5)