FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-3357-CHX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26699469
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT626070.23448780947666037No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA506470.18969291112119122No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG309970.11609594183315032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG184850.026.6595575
GACTCTA186350.026.6323834
CTGACTC198400.024.7142052
TGACTCT209450.023.561273
CTCTAGC217700.022.5888086
TAGCAGA230150.021.4586329
GCTGACT230150.021.427011
TCTATCG225850.021.17022916
AGCTGAC181950.020.54381
CTATCGA238450.020.0663217
CTAGCAG253450.020.038168
TATCGAT251150.019.06541418
GATCTAT262150.018.54552714
AGATCTA286400.016.99968713
ATCTATC286400.016.9631515
ATCGATT301600.016.07351719
CGATTCT301700.016.01030221
TCGATTC322150.015.04818520
TTCTAGC322150.015.04801624
TAGCATG329250.014.68077527
Produced by FastQC (version 0.11.5)