FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACACACAAGCACAAATTGTGAACTGCGTGACGCCGCGAGGGGTTAAG11.5625No Hit
CACTCTGCTGGGCCGACACTGACACTGAGAGACGAAAGCTAGGGGAGCAA11.5625No Hit
GATGAAGTCGGTGAGGCTGTGCAATATCGACTGCTTCAAAGTGACAGCAA11.5625No Hit
CGGCTCAGTGCTGGTGGCTGACGGCACGCACAGTGGTAGTTGCTGACTTT11.5625No Hit
GGAACTTACATGCCCTACTCTATTGAACAACGAAAAGGAGGGGGGATCGA11.5625No Hit
CTAAGATGGACCAAAAGCAATACTGGGCTGGTGATCAAAACAAGTATCAG11.5625No Hit
CTGGCTTGCTTCCCAGCAAGGCCACTAGTTGCGTGCCAATTGTATCTGAG11.5625No Hit
CAGCTTGTCCCTTGCCCAACTTAGGATTTGAGCCAAGGTCTTGGTTAGTG11.5625No Hit
TTTAGTAAATGCAGCTACATAAAAATAGTGCTTGATGTTATTTTCACAAT11.5625No Hit
CTCACCCCCAGTCAGTTGCAATGCTCGAAGCAAACCCATGAGCTATTCCC11.5625No Hit
CCCGCGTCCACCCGGTGGTCTCGTTCTTCCCTCTTCCTCGTATTTCTGTT11.5625No Hit
CGCCGACCCCAACAGGGTGGCGCAGGACGCCGTGATCGCGTTCAAGACGG11.5625No Hit
CCGCACCCCAGATCTCCGAACCCTAACCCCTTGCACATGTTATTGCACTG11.5625No Hit
CTCGGTCTTCCGGAAAGCCTGCTGCTCCCTTATCGGTTACAACATAGTGA11.5625No Hit
CTCTACTCCTCTTCCTCCTCTCTCTACTCTCCAAGTTCTCTTCTCCGTCG11.5625No Hit
GGAGTCACAGACCATCGTTAGCTGGGAATCTACCCCCCAGTGCTCTTAAT11.5625No Hit
GAACTTCACGGTGCTCGGCGGCAACTCCATGGTGCAGGTGAACGCCAACA11.5625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.5625No Hit
CCGAAATGCAAGTTCTTCCTTGGCTTGCTGCCCTTCAGAGATGCTGGACA11.5625No Hit
GGACCTCCTAGTAGGGAACATCCCTGAAGAGGTTCGGATGCTTGCTGATG11.5625No Hit
GCCATGAACAAGCTCAGCACCAAGAAGCCGTGGTCCCTGTCTTTCTCCTT11.5625No Hit
CATAATCCTGTTAGAATTTCTGTGCGTCGTTCATATATTCTTGAGGACAC11.5625No Hit
CTACAATCAGATGGGCGCGGCGTACGGTGGCCAGCAGCCATACGGTGGAT11.5625No Hit
CAATGAGCCTGAACCTCCAGCGCGTAGTAAGAGTGATGGGGGACTTTACA11.5625No Hit
TGATGGCGATAACTGGAGTGCTGGACAGCGTCAGCTCTTCTGTCTTGGCA11.5625No Hit
TTGTACTACGTGGATGCCAGCTTAAAAACACTGAATGGGTTATTGGGGTT11.5625No Hit
GAAAGCTGAATCGAGCCCCCTCCCGACTTAGAGTGAAGGGAAAAGCAGGT11.5625No Hit
CTTGGCTCCTGAATACGCGATCCGAGGCCAAGTGACGAAGAAGTCCGATA11.5625No Hit
ATTGACATGGAATCCTGGCCTGATCTTCCTTCCCTGAATCCTACACTTGA11.5625No Hit
TTTGCATGCCAATTTTTGAAGGGTTCAGCAGTTATGTAGCGCTGTTTTTT11.5625No Hit
CTTAATTCCTGCAAGATTGGGTACTAGACAATTTATAAGGCGGCAACCAT11.5625No Hit
CAAGGCTGGAGTTGAGTGAGGTCTTGATGGATCATGGAGCTATCAAGATA11.5625No Hit
GGGGAACAGCCCTCTCGGATGGAACCACTTCCTGATGGCTCAATGAATCC11.5625No Hit
TATGTTGGCACCAGATTTGCCGTTATGTGCCATTGGTGATGAAAAGAGAC11.5625No Hit
AAGATGCATCTTTGACACATTTGAGCCAGTCTCAGTAGGGCAGCTATGTT11.5625No Hit
GGATCAACTCATTGCCCACCAGATGGAAATCCTCTGCTCGGTTTACCATC11.5625No Hit
TGCCTCTTGGAAAAAGAGGAAACCAAATCATTGTTCAGTGCTGATGCAAT11.5625No Hit
GTTGAAAGAGATAAACTACAATGCTGCAGCAATGGATGAAAGCACTGAGC11.5625No Hit
CCGTAGCTAGCTAGTACGTAGTTGCCGGCGAACAAGGAGGAGCGAGCTTG11.5625No Hit
AGAAGGTTAAGTTCAACACTCCTTAGGTGTTCGATGATATGCTCGAACTG11.5625No Hit
CGTGCTTACGTTCGCCTTCGTCTGCGCACTGGAGATCACCTACGTCGCGC11.5625No Hit
CTCGCGTATCTACCGCTCGCTCAACAGACTCAGATAATACAATGTCATCA11.5625No Hit
GCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATT11.5625No Hit
CTGGGCGCATGCAGTCCCCGATCGACCTCGCCCGCGAGCGCGTCTCGCTG11.5625No Hit
GACGTAAACGCCTTGCTGAACAAAGGCATGGCCGCGGACAGCCGCTTCCT11.5625No Hit
CGGAGCTCGGCGCGCTCATGGGCCTCACCGTGCTCGACCTCAGCTTCAAC11.5625No Hit
CGGAATGCTCGAGCTGCTCGCGCGGCGAGAGCGGGCCGCCGCGTGCCGGC11.5625No Hit
AATCTTTGTCGCTGAGGCAACTTGTGCATTTCGCTTGTGAAACGGTTGTG11.5625No Hit
CTGCCAGTGGAAAACCTCACAAGGCATCTCCTGCTGACGATGATCACATG11.5625No Hit
TTTTTTTTTTTTTTTTTTTAAAGTTAAAAAAGATTTTTTGCCATAAGAAA11.5625No Hit
CTACCACCCGCTCCTCTCCCCGTCGCCGCCGCACTTTCCCCTGCCGCCCC11.5625No Hit
AACAAATATAATTTTCCTGTATTTCTACTTTCAGAAGAGAGTACAAACAC11.5625No Hit
CCGCACTGCCTTGCCCCCTACTCTGCCTCGCTTAGCTTAGCCGCCGCTGC11.5625No Hit
GGCAGTTAAAAATTCAGAACTAATTAAAGAAAGCTCGAACAATAGTGTCC11.5625No Hit
TTGAACTTGGATGAGAGCAAAGTACGTCGTGCTGGACCTGCTGAGAATGT11.5625No Hit
GTCCTTTATATAGTTTTCTACCGCTTTAATAGCTTCATTCTGTGTTTTTG11.5625No Hit
GATTGAAGAGAAAGATGAATCTCATGGATACTGGTAATGGAGCAAATCTG11.5625No Hit
TAGGCCTTGGTGGGGCTGCTGGGAGAAAGTCCCCTCCCTTTTGTCTACTT11.5625No Hit
CGTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGC11.5625No Hit
GTTCCGGATGTGGTTCGACCCCACGGCGGCCTTCCACGCCTACTCCGTGC11.5625No Hit
CCAGGAAGGCGATGAACTCCCTGAAGTTCTCCTCGGTCGGGAGGTAGTGG11.5625No Hit
GATGATGTGGATCTGATGATCATTGCAAGGGGAACTCCTGGTTTCTCAGG11.5625No Hit
CTCTTTACTGACGACAAACAGGTTATAGTTTACTAACCTTCTTGATCTCC11.5625No Hit
TCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACC11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG50.070.048
TTATGAC50.070.070
GTTGATT50.070.02
TGGTTGA50.070.030
CCGGATT50.070.065
TGGTCAT50.070.017
CCGTGTG50.070.058
TTCACAC50.070.07
GTTGAAA50.070.032
GGTTGAA50.070.031
GTCATCG50.070.019
CACACAA50.070.09
AGCCAGT50.070.039
CCAGTGG50.070.041
GGCGCGA50.070.046
TGCCGGA50.070.063
CACAATT50.070.011
GAAGCTA50.070.051
AGCTACC50.070.053
ATTGGTC50.070.015
Produced by FastQC (version 0.11.5)