FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23204840
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT524420.22599595601607253No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA416010.17927725422799726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261410.11265322234499354No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG252840.10896002730464852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA147750.026.531424
ACTCTAG153000.025.4382975
CTGACTC162150.023.958992
TGACTCT175750.022.4040343
CTCTAGC181350.021.55866
AGCTGAC150850.020.9742571
GCTGACT186450.020.8740061
TAGCAGA193050.020.5604179
TCTATCG196900.019.234416
CTAGCAG213600.018.9263788
CTATCGA210100.018.15911717
TATCGAT217700.017.6054918
GATCTAT232600.016.49282814
AGATCTA246950.015.53444813
ATCTATC256250.015.052649515
ATCGATT263500.014.67824419
CGATTCT268650.014.18831121
TCGATTC277950.014.01586620
TCTAGCA434550.013.3626477
TTCTAGC290850.013.26164624
Produced by FastQC (version 0.11.5)