FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23689895
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT602660.25439538672501505No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA478520.20199329714209371No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG264340.11158344095657663No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA245740.10373199205821722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA167100.027.438464
ACTCTAG176900.025.8398695
CTGACTC194350.023.5547222
AGCTGAC155200.023.1598281
TGACTCT202800.022.8497893
CTCTAGC201500.022.8421336
GCTGACT207350.022.0273931
TAGCAGA216650.021.471279
CTAGCAG232400.020.2269948
TCTATCG227500.019.6784416
CTATCGA238900.018.66607517
TATCGAT252600.017.76448218
GATCTAT266900.016.93061614
AGATCTA281950.016.0019613
ATCTATC287700.015.76757215
ATCGATT295100.015.26530219
CGATTCT298100.015.06464521
TCGATTC304850.014.71960220
TTCTAGC320150.013.98308624
TAGCATG328750.013.74490827
Produced by FastQC (version 0.11.5)