FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n02_ZmTARGET.0808-183-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25998523
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT790260.3039634213066642No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAA599120.2304438602146745No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG362530.13944253679334015No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA298860.1149526840428589No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291430.11209482938703864No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA267710.1029712341735721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA209100.029.2581924
ACTCTAG220200.027.8475445
CTGACTC229350.026.6130372
TGACTCT242750.025.4186423
AGCTGAC186050.025.226191
CTCTAGC245100.025.0190586
GCTGACT249250.024.7413441
TAGCAGA262300.023.592429
CTAGCAG282250.022.1603078
TCTATCG276300.021.81490316
CTATCGA290550.020.80518717
TATCGAT303300.019.9652118
GATCTAT307100.019.77481314
AGATCTA322100.018.84297213
ATCTATC332600.018.3957615
ATCGATT353550.017.25626419
CGATTCT361650.016.73420321
TCGATTC376000.016.1700220
TTCTAGC379250.015.8833524
AGCAGAT412650.015.46310310
Produced by FastQC (version 0.11.5)