FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21669309
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG402730.18585271916146473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA91900.054.6408716
ACGTCTG96800.052.09158715
CACGTCT100650.050.7942214
CACACGT109150.048.0571712
ATCGGAA117800.046.4650232
GATCGGA111700.046.303371
TCGGAAG115350.045.7368133
GCACACG121250.043.1172611
CAGTCAC92000.042.3829127
ACACGTC126600.041.32240313
CGGAAGA135050.039.8404164
CTGAACT138000.038.3136419
AGCACAC143550.038.0270310
AGAGCAC140500.037.8575638
CCAGTCA113650.036.0940326
CTCCAGT128650.035.3375424
AACTCCA177350.031.03574822
AGTCACC53900.029.8483228
TCTGAAC179150.029.6303418
GTCTGAA188850.027.96009317
Produced by FastQC (version 0.11.5)