FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21377342
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT333900.15619341263287082No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC306800.14351643904092473No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG286030.13380054452045537No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG285170.1333982494175375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCGGGGGTGG274460.12838827203120012TruSeq Adapter, Index 7 (97% over 35bp)
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC268620.12565640761138594No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT230310.10773556413140606No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCGGGGTTTG226840.10611235016963287TruSeq Adapter, Index 7 (97% over 35bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA221110.10343194210019188No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC218350.10214085549082763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA217700.058.08648316
ACGTCTG222100.057.0141315
ATCGGGG164650.056.13905740
GTCACTA224050.055.6923329
ATCGGAA229050.055.3517652
GATCGGA229600.055.020721
TCACTAC228400.054.64696530
CACGTCT232600.054.56091314
TCGGAAG232750.054.5322533
AGTCACT231750.053.7969928
ACTACAG233950.053.48271632
CATCGGG195850.052.7177539
CACTACA241100.051.98374631
CTACAGC244250.051.24180633
GTCTGAA248500.051.07012617
CACACGT249100.050.96176512
CAGTCAC248100.050.1386327
GCATCGG215700.049.13208438
GCACACG266500.047.7790111
CGGAAGA271800.047.0068054
Produced by FastQC (version 0.11.5)