FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21377342
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT333900.15619341263287082No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC306800.14351643904092473No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG286030.13380054452045537No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG285170.1333982494175375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCGGGGGTGG274460.12838827203120012TruSeq Adapter, Index 7 (97% over 35bp)
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC268620.12565640761138594No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT230310.10773556413140606No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCGGGGTTTG226840.10611235016963287TruSeq Adapter, Index 7 (97% over 35bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA221110.10343194210019188No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC218350.10214085549082763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA217600.058.0970816
ACGTCTG221900.057.0181915
ATCGGGG163950.056.20795440
GTCACTA224550.055.53726629
ATCGGAA228300.055.4722632
GATCGGA229200.055.040371
TCGGAAG231650.054.7156373
CACGTCT231950.054.6685314
TCACTAC228150.054.66093430
AGTCACT231300.053.8563728
ACTACAG234000.053.44136432
CATCGGG194900.052.79512439
CACTACA241100.051.94018631
CTACAGC244000.051.27995333
GTCTGAA247950.051.14105617
CACACGT249200.050.89916612
CAGTCAC248800.049.9554727
GCATCGG215900.048.9244538
GCACACG266350.047.79276711
CGGAAGA272500.046.821824
Produced by FastQC (version 0.11.5)