Basic Statistics
Measure | Value |
---|---|
Filename | HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-02.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 18113315 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC | 129472 | 0.7147890929959535 | TruSeq Adapter, Index 3 (97% over 36bp) |
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT | 35345 | 0.19513269658259685 | No Hit |
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC | 33667 | 0.1858687932054403 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 29853 | 0.16481245978441825 | No Hit |
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT | 28604 | 0.15791697985708303 | No Hit |
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG | 26901 | 0.14851505646536817 | No Hit |
CTCATCTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTC | 24299 | 0.13414993335013498 | No Hit |
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC | 23817 | 0.13148890746944997 | No Hit |
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC | 23164 | 0.12788382468918583 | No Hit |
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT | 20347 | 0.11233172944875082 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC | 19747 | 0.10901924909935039 | No Hit |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA | 18447 | 0.10184220834231614 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTAATCG | 17355 | 0.0 | 61.972713 | 33 |
TCGTATG | 17565 | 0.0 | 60.41451 | 43 |
CGTATGC | 17675 | 0.0 | 59.998917 | 44 |
TATGCCG | 17760 | 0.0 | 59.55394 | 46 |
CGTCTGA | 18515 | 0.0 | 59.03534 | 16 |
TAATCGA | 18160 | 0.0 | 58.97503 | 34 |
CTCGTAT | 16950 | 0.0 | 58.043198 | 42 |
ATCGGAA | 19025 | 0.0 | 57.642006 | 2 |
ACGTCTG | 19335 | 0.0 | 56.621838 | 15 |
GTCACTA | 19035 | 0.0 | 56.504814 | 29 |
GATCGGA | 19360 | 0.0 | 56.246513 | 1 |
GTATGCC | 18940 | 0.0 | 55.99144 | 45 |
CACTAAT | 19315 | 0.0 | 55.882866 | 31 |
TCGGAAG | 19640 | 0.0 | 55.7662 | 3 |
ACTAATC | 19330 | 0.0 | 55.73132 | 32 |
TCACTAA | 19595 | 0.0 | 55.032875 | 30 |
CACGTCT | 20115 | 0.0 | 54.44391 | 14 |
AGTCACT | 19820 | 0.0 | 54.17871 | 28 |
AATCGAT | 19445 | 0.0 | 53.961784 | 35 |
GTCTGAA | 20710 | 0.0 | 52.812138 | 17 |