FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18113315
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC1294720.7147890929959535TruSeq Adapter, Index 3 (97% over 36bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT353450.19513269658259685No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC336670.1858687932054403No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG298530.16481245978441825No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT286040.15791697985708303No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG269010.14851505646536817No Hit
CTCATCTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTC242990.13414993335013498No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC238170.13148890746944997No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC231640.12788382468918583No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT203470.11233172944875082No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC197470.10901924909935039No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA184470.10184220834231614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG173550.061.97271333
TCGTATG175650.060.4145143
CGTATGC176750.059.99891744
TATGCCG177600.059.5539446
CGTCTGA185150.059.0353416
TAATCGA181600.058.9750334
CTCGTAT169500.058.04319842
ATCGGAA190250.057.6420062
ACGTCTG193350.056.62183815
GTCACTA190350.056.50481429
GATCGGA193600.056.2465131
GTATGCC189400.055.9914445
CACTAAT193150.055.88286631
TCGGAAG196400.055.76623
ACTAATC193300.055.7313232
TCACTAA195950.055.03287530
CACGTCT201150.054.4439114
AGTCACT198200.054.1787128
AATCGAT194450.053.96178435
GTCTGAA207100.052.81213817