FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18113315
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC1294720.7147890929959535TruSeq Adapter, Index 3 (97% over 36bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT353450.19513269658259685No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC336670.1858687932054403No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG298530.16481245978441825No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT286040.15791697985708303No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG269010.14851505646536817No Hit
CTCATCTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTC242990.13414993335013498No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC238170.13148890746944997No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC231640.12788382468918583No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT203470.11233172944875082No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC197470.10901924909935039No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA184470.10184220834231614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG173450.061.98828533
TCGTATG174850.060.63089443
CGTATGC176300.060.11237344
TATGCCG177550.059.55101446
CGTCTGA184650.059.15730716
TAATCGA181300.058.99541534
CTCGTAT169450.057.9983842
ATCGGAA190100.057.6505242
ACGTCTG193350.056.6399615
GTCACTA190550.056.3904229
GATCGGA193650.056.213781
GTATGCC189200.056.03214645
CACTAAT192650.055.97341531
ACTAATC192750.055.8540532
TCGGAAG196050.055.829913
TCACTAA195950.054.97931330
CACGTCT201350.054.40723414
AGTCACT198000.054.18042428
AATCGAT193950.053.99263835
GTCTGAA207150.052.79940417
Produced by FastQC (version 0.11.5)