FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47980517
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC765460.15953558816383742TruSeq Adapter, Index 6 (97% over 36bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT654350.1363782720390445No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA546110.11381911537134959No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT544010.11338143771981449No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC522790.10895880926001693No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG501790.10458203274466592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG238600.051.02083628
CGTCTGA256400.047.88537216
ATCGGAA269650.045.770652
ACGTCTG269550.045.70499815
GTCACGA276750.044.21503429
GATCGGA282750.043.55081
TCGGAAG289150.042.853533
TCGTATG158700.042.5638843
CACGTCT291400.042.31389214
CGTATGC158500.042.13179444
TATGCCG158350.041.66312446
CTCGTAT164950.039.06262242
CACACGT318650.038.9378712
GTCTGAA319100.038.83831417
CAGTCAC318750.038.4880427
TCACGAC323800.038.10381330
ACGACAT324500.037.27511235
GTATGCC198350.034.67280245
GCACACG376000.033.38056611
CGGAAGA387150.032.521564