Basic Statistics
Measure | Value |
---|---|
Filename | HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-01.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 47980517 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC | 76546 | 0.15953558816383742 | TruSeq Adapter, Index 6 (97% over 36bp) |
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT | 65435 | 0.1363782720390445 | No Hit |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA | 54611 | 0.11381911537134959 | No Hit |
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT | 54401 | 0.11338143771981449 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC | 52279 | 0.10895880926001693 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 50179 | 0.10458203274466592 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGTCACG | 23860 | 0.0 | 51.020836 | 28 |
CGTCTGA | 25640 | 0.0 | 47.885372 | 16 |
ATCGGAA | 26965 | 0.0 | 45.77065 | 2 |
ACGTCTG | 26955 | 0.0 | 45.704998 | 15 |
GTCACGA | 27675 | 0.0 | 44.215034 | 29 |
GATCGGA | 28275 | 0.0 | 43.5508 | 1 |
TCGGAAG | 28915 | 0.0 | 42.85353 | 3 |
TCGTATG | 15870 | 0.0 | 42.56388 | 43 |
CACGTCT | 29140 | 0.0 | 42.313892 | 14 |
CGTATGC | 15850 | 0.0 | 42.131794 | 44 |
TATGCCG | 15835 | 0.0 | 41.663124 | 46 |
CTCGTAT | 16495 | 0.0 | 39.062622 | 42 |
CACACGT | 31865 | 0.0 | 38.93787 | 12 |
GTCTGAA | 31910 | 0.0 | 38.838314 | 17 |
CAGTCAC | 31875 | 0.0 | 38.48804 | 27 |
TCACGAC | 32380 | 0.0 | 38.103813 | 30 |
ACGACAT | 32450 | 0.0 | 37.275112 | 35 |
GTATGCC | 19835 | 0.0 | 34.672802 | 45 |
GCACACG | 37600 | 0.0 | 33.380566 | 11 |
CGGAAGA | 38715 | 0.0 | 32.52156 | 4 |