Basic Statistics
Measure | Value |
---|---|
Filename | HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-01-.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 47980517 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC | 76546 | 0.15953558816383742 | TruSeq Adapter, Index 6 (97% over 36bp) |
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT | 65435 | 0.1363782720390445 | No Hit |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA | 54611 | 0.11381911537134959 | No Hit |
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT | 54401 | 0.11338143771981449 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC | 52279 | 0.10895880926001693 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 50179 | 0.10458203274466592 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGTCACG | 23940 | 0.0 | 50.96731 | 28 |
CGTCTGA | 25660 | 0.0 | 47.943535 | 16 |
ATCGGAA | 26975 | 0.0 | 45.844185 | 2 |
ACGTCTG | 27125 | 0.0 | 45.54759 | 15 |
GTCACGA | 27645 | 0.0 | 44.32633 | 29 |
GATCGGA | 28295 | 0.0 | 43.630993 | 1 |
TCGGAAG | 28900 | 0.0 | 42.936028 | 3 |
TCGTATG | 15975 | 0.0 | 42.415573 | 43 |
CACGTCT | 29260 | 0.0 | 42.25998 | 14 |
CGTATGC | 16010 | 0.0 | 41.907494 | 44 |
TATGCCG | 15870 | 0.0 | 41.791782 | 46 |
CACACGT | 31895 | 0.0 | 38.967175 | 12 |
CTCGTAT | 16640 | 0.0 | 38.869473 | 42 |
GTCTGAA | 32050 | 0.0 | 38.75603 | 17 |
TCACGAC | 32180 | 0.0 | 38.4494 | 30 |
CAGTCAC | 32045 | 0.0 | 38.360325 | 27 |
ACGACAT | 32330 | 0.0 | 37.43516 | 35 |
GTATGCC | 19850 | 0.0 | 34.770027 | 45 |
GCACACG | 37335 | 0.0 | 33.683228 | 11 |
CGGAAGA | 38690 | 0.0 | 32.61475 | 4 |