FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHXDEX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47980517
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC765460.15953558816383742TruSeq Adapter, Index 6 (97% over 36bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT654350.1363782720390445No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA546110.11381911537134959No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT544010.11338143771981449No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC522790.10895880926001693No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG501790.10458203274466592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG239400.050.9673128
CGTCTGA256600.047.94353516
ATCGGAA269750.045.8441852
ACGTCTG271250.045.5475915
GTCACGA276450.044.3263329
GATCGGA282950.043.6309931
TCGGAAG289000.042.9360283
TCGTATG159750.042.41557343
CACGTCT292600.042.2599814
CGTATGC160100.041.90749444
TATGCCG158700.041.79178246
CACACGT318950.038.96717512
CTCGTAT166400.038.86947342
GTCTGAA320500.038.7560317
TCACGAC321800.038.449430
CAGTCAC320450.038.36032527
ACGACAT323300.037.4351635
GTATGCC198500.034.77002745
GCACACG373350.033.68322811
CGGAAGA386900.032.614754