FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27962279
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC824110.29472204322115514TruSeq Adapter, Index 2 (97% over 36bp)
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC630110.22534286279026114No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG615500.22011796677946027No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA601470.21510049306066933No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT592770.21198915868052098No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT550610.19691170379925038No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG514280.1839192005773206No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC478070.17096961231235838No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC378740.13544675668245781No Hit
GCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGT360260.12883785330945308No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT324770.11614575478629621No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT288110.10303523543270562No Hit
TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC280650.10036735560788876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA164150.052.6432416
TATGCCG121950.051.88889346
TCGTATG128450.051.6335643
CGTATGC126650.050.95813444
ATCGGAA170250.050.783692
ACGTCTG171400.050.416515
CTCGTAT133500.049.62786542
AATCTCG151300.048.71645439
GATCGGA177450.048.6436421
TCGGAAG178900.048.445993
CACGTCT183050.047.16956314
GTATGCC139100.046.7494345
AGTCACC183100.045.8788528
GTCTGAA192050.045.1048617
CAGTCAC196900.042.8943127
CACACGT205550.042.1092612
GTCACCC199500.042.0545729
ACCCAAC204000.041.31289732
TCACCCA208300.040.46273830
TCTCGTA177000.038.14303241
Produced by FastQC (version 0.11.5)