FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27962279
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC824110.29472204322115514TruSeq Adapter, Index 2 (97% over 36bp)
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC630110.22534286279026114No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG615500.22011796677946027No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA601470.21510049306066933No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT592770.21198915868052098No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT550610.19691170379925038No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG514280.1839192005773206No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC478070.17096961231235838No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC378740.13544675668245781No Hit
GCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGT360260.12883785330945308No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT324770.11614575478629621No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT288110.10303523543270562No Hit
TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC280650.10036735560788876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA163450.052.5903516
TATGCCG121050.051.75418546
TCGTATG127800.051.5674843
CGTATGC126050.050.75636744
ATCGGAA169700.050.638822
ACGTCTG170050.050.5492115
CTCGTAT133050.049.5326942
AATCTCG150500.048.6266139
GATCGGA176900.048.5178031
TCGGAAG178400.048.2873953
CACGTCT182050.047.1787614
GTATGCC138100.046.63171445
AGTCACC181900.045.89290228
GTCTGAA190850.045.1317417
CAGTCAC196600.042.71053327
GTCACCC198300.042.0443229
CACACGT204900.042.02072512
ACCCAAC203000.041.27505532
TCACCCA206250.040.59340730
TCTCGTA177200.037.9617441
Produced by FastQC (version 0.11.5)