FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37387115
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT590830.15803038025266192No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA498720.1333935501575877No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC482100.1289481683729809No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT462670.12375119075114516No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG450490.12049338388372571No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC416050.11128165412067767No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG403280.10786603887462298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA216800.049.98119416
ACGTCTG219950.049.24934415
ATCGGAA227850.047.39262
GATCGGA231350.046.5241361
CACGTCT236550.046.05951314
TCGGAAG240000.045.2123033
TATCGGG63700.043.1309839
AGTCACC248600.042.87131528
CACACGT257200.042.4165612
CATTTTA253950.042.0074634
GTCTGAA268200.040.57198717
CAGTCAC270300.039.77917527
GCACACG301550.036.25950211
CGGAAGA306500.035.916884
TATCGCG55900.035.124539
ACACGTC312650.035.0840813
TTTTATC279600.035.0368836
TTTATCG141900.034.82667537
AGCACAC326250.033.63252610
ATTTTAT317150.033.4488335