FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20880415
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATGGGGGGGGG392150.18780756991659409TruSeq Adapter, Index 12 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA356310.1706431601096051No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT343920.16470937000054836No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC319050.1527986871908437No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG313060.14992997026160637No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG299480.14342626810817696No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT296000.14175963456665014No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC256590.1228854886265431No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATGGGGGGTTG229580.10994992197233629TruSeq Adapter, Index 12 (97% over 37bp)
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC214100.10253627621864794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA206000.058.39506516
ATCGGAA210250.057.2866022
ACGTCTG213200.056.39002615
GATCGGA215700.055.6923141
CACGTCT217200.055.36778614
ATGGGGG169400.055.18475340
TCGGAAG221300.054.5052383
GTCTGAA225150.053.42817717
AGTCACC220150.053.29335428
CACACGT236600.050.872912
CAGTCAC231900.050.81934727
CATGGGG203600.046.89485539
GCATGGG205700.046.82457438
CGGAAGA261300.046.496764
TCTGAAC260100.046.4779418
GCACACG265200.045.54519711
AGCACAC266500.045.3758810
ACACGTC270500.044.7301213
TCCAGTC270350.044.0838525
CCAGTCA268950.043.97495726
Produced by FastQC (version 0.11.5)