FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-01-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-6435-CHX-01-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20880415
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATGGGGGGGGG392150.18780756991659409TruSeq Adapter, Index 12 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA356310.1706431601096051No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT343920.16470937000054836No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC319050.1527986871908437No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG313060.14992997026160637No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG299480.14342626810817696No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT296000.14175963456665014No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC256590.1228854886265431No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATGGGGGGTTG229580.10994992197233629TruSeq Adapter, Index 12 (97% over 37bp)
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC214100.10253627621864794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA205800.058.4858916
ATCGGAA210400.057.2287752
ACGTCTG212550.056.57900615
GATCGGA215550.055.7147641
CACGTCT216900.055.44442414
ATGGGGG169400.055.18481440
TCGGAAG221000.054.5313763
GTCTGAA225100.053.45565417
AGTCACC220050.053.36522328
CAGTCAC231500.050.9524527
CACACGT237000.050.80185712
CATGGGG203800.046.86606239
GCATGGG205750.046.8642738
CGGAAGA261300.046.4830864
TCTGAAC260400.046.42444618
GCACACG265400.045.5371911
AGCACAC265900.045.47821410
ACACGTC270350.044.76793713
TCCAGTC271350.043.9600325
CCAGTCA269750.043.8834126
Produced by FastQC (version 0.11.5)