FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27419429
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT412020.15026571122250576No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC364390.1328948170291949No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG357170.13026164768055526No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA327890.11958308832762345No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT313710.11441157290328692No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC306010.11160334520459926No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG277850.10133325533511292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA207200.054.35791416
ATCGGAA211750.053.426952
ACGTCTG213850.052.8145815
GATCGGA219500.051.428481
TCGGAAG222100.051.1423843
CACGTCT225900.049.92004814
AGTCACC231000.047.894928
CACACGT242600.046.4846112
GTCTGAA243800.046.34092717
AGGCGAT232350.045.68638235
CAGTCAC245000.045.2437727
CGGAAGA273050.042.125334
GCACACG272550.041.5951211
AGCACAC281800.040.35419510
ACCAGGC275650.040.1350232
ACACGTC289350.039.40938613
TCTGAAC288800.039.3262418
GAGCACA296250.038.6576739
CCAGTCA298250.037.30677426
TCCAGTC304700.036.7697625
Produced by FastQC (version 0.11.5)