FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-03-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-03-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27419429
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT412020.15026571122250576No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC364390.1328948170291949No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG357170.13026164768055526No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA327890.11958308832762345No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT313710.11441157290328692No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC306010.11160334520459926No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG277850.10133325533511292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA206550.054.4103316
ATCGGAA211550.053.4280852
ACGTCTG213450.052.81514715
GATCGGA218000.051.67021
TCGGAAG221800.051.1644673
CACGTCT225900.049.81157314
AGTCACC231600.047.61976228
CACACGT241300.046.61889612
GTCTGAA243800.046.25477217
AGGCGAT231900.045.68445235
CAGTCAC245450.045.0325427
CGGAAGA272850.042.0922784
GCACACG272200.041.55849511
AGCACAC281800.040.2795810
ACCAGGC274850.040.13721532
ACACGTC287950.039.51581213
TCTGAAC287400.039.43254518
GAGCACA296100.038.5944189
CCAGTCA299250.037.11197726
TCCAGTC304700.036.67791425
Produced by FastQC (version 0.11.5)