FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21952154
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC666110.3034371934526334TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT418720.19074210211899933No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA362910.16531862886894835No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG333850.1520807479758023No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC317620.14468739605234185No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC313660.14288347284735703No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT281220.12810587972369364No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG262090.11939147292789583No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC240650.10962477759585687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA194600.056.88816
ACGTCTG197350.056.20143515
ATCGGAA199850.055.5208362
GATCGGA199750.055.3209571
ACTCAAT198600.054.36698535
TCGGAAG207200.053.7035063
CACGTCT208800.053.13614714
TCGTATG108800.052.24207743
AGTCACC212750.051.31247328
GTCTGAA216300.051.2455117
CGTATGC108150.051.1643744
CTCGTAT116800.051.06085642
TATGCCG107050.050.97083346
CACTCAA217700.050.33661334
CCACTCA222350.049.34733
CACACGT227500.048.76925312
CAGTCAC226600.048.14542427
AATCTCG151100.047.71600739
ACCACTC236900.046.31619332
GTATGCC123200.046.13575445