Basic Statistics
Measure | Value |
---|---|
Filename | HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-02.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 21952154 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC | 66611 | 0.3034371934526334 | TruSeq Adapter, Index 7 (97% over 35bp) |
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT | 41872 | 0.19074210211899933 | No Hit |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA | 36291 | 0.16531862886894835 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 33385 | 0.1520807479758023 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC | 31762 | 0.14468739605234185 | No Hit |
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC | 31366 | 0.14288347284735703 | No Hit |
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT | 28122 | 0.12810587972369364 | No Hit |
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG | 26209 | 0.11939147292789583 | No Hit |
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC | 24065 | 0.10962477759585687 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCTGA | 19460 | 0.0 | 56.888 | 16 |
ACGTCTG | 19735 | 0.0 | 56.201435 | 15 |
ATCGGAA | 19985 | 0.0 | 55.520836 | 2 |
GATCGGA | 19975 | 0.0 | 55.320957 | 1 |
ACTCAAT | 19860 | 0.0 | 54.366985 | 35 |
TCGGAAG | 20720 | 0.0 | 53.703506 | 3 |
CACGTCT | 20880 | 0.0 | 53.136147 | 14 |
TCGTATG | 10880 | 0.0 | 52.242077 | 43 |
AGTCACC | 21275 | 0.0 | 51.312473 | 28 |
GTCTGAA | 21630 | 0.0 | 51.24551 | 17 |
CGTATGC | 10815 | 0.0 | 51.16437 | 44 |
CTCGTAT | 11680 | 0.0 | 51.060856 | 42 |
TATGCCG | 10705 | 0.0 | 50.970833 | 46 |
CACTCAA | 21770 | 0.0 | 50.336613 | 34 |
CCACTCA | 22235 | 0.0 | 49.347 | 33 |
CACACGT | 22750 | 0.0 | 48.769253 | 12 |
CAGTCAC | 22660 | 0.0 | 48.145424 | 27 |
AATCTCG | 15110 | 0.0 | 47.716007 | 39 |
ACCACTC | 23690 | 0.0 | 46.316193 | 32 |
GTATGCC | 12320 | 0.0 | 46.135754 | 45 |