FastQCFastQC Report
Thu 27 Sep 2018
HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-02-.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-02-.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21952154
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC666110.3034371934526334TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT418720.19074210211899933No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA362910.16531862886894835No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG333850.1520807479758023No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC317620.14468739605234185No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC313660.14288347284735703No Hit
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT281220.12810587972369364No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG262090.11939147292789583No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC240650.10962477759585687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA195050.057.07972716
ACGTCTG198050.056.32087715
ATCGGAA200250.055.6546332
GATCGGA200400.055.4035031
ACTCAAT199400.054.41213235
TCGGAAG207900.053.775343
CACGTCT209300.053.31019614
TCGTATG108450.052.4752143
AGTCACC213150.051.4624828
CGTATGC108350.051.3283344
GTCTGAA217200.051.32320817
TATGCCG107450.051.10680446
CTCGTAT116900.051.0770442
CACTCAA217500.050.55989534
CCACTCA223150.049.38965233
CACACGT227800.048.9354812
CAGTCAC227500.048.20110727
AATCTCG150850.047.9110839
GTATGCC122900.046.50466545
ACCACTC238000.046.337432