Basic Statistics
Measure | Value |
---|---|
Filename | HMGFWBGX7_n01_ZmTARGET.0830-183-CHXDEX-02-.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 21952154 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC | 66611 | 0.3034371934526334 | TruSeq Adapter, Index 7 (97% over 35bp) |
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT | 41872 | 0.19074210211899933 | No Hit |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA | 36291 | 0.16531862886894835 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 33385 | 0.1520807479758023 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC | 31762 | 0.14468739605234185 | No Hit |
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC | 31366 | 0.14288347284735703 | No Hit |
CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT | 28122 | 0.12810587972369364 | No Hit |
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG | 26209 | 0.11939147292789583 | No Hit |
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC | 24065 | 0.10962477759585687 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCTGA | 19505 | 0.0 | 57.079727 | 16 |
ACGTCTG | 19805 | 0.0 | 56.320877 | 15 |
ATCGGAA | 20025 | 0.0 | 55.654633 | 2 |
GATCGGA | 20040 | 0.0 | 55.403503 | 1 |
ACTCAAT | 19940 | 0.0 | 54.412132 | 35 |
TCGGAAG | 20790 | 0.0 | 53.77534 | 3 |
CACGTCT | 20930 | 0.0 | 53.310196 | 14 |
TCGTATG | 10845 | 0.0 | 52.47521 | 43 |
AGTCACC | 21315 | 0.0 | 51.46248 | 28 |
CGTATGC | 10835 | 0.0 | 51.32833 | 44 |
GTCTGAA | 21720 | 0.0 | 51.323208 | 17 |
TATGCCG | 10745 | 0.0 | 51.106804 | 46 |
CTCGTAT | 11690 | 0.0 | 51.07704 | 42 |
CACTCAA | 21750 | 0.0 | 50.559895 | 34 |
CCACTCA | 22315 | 0.0 | 49.389652 | 33 |
CACACGT | 22780 | 0.0 | 48.93548 | 12 |
CAGTCAC | 22750 | 0.0 | 48.201107 | 27 |
AATCTCG | 15085 | 0.0 | 47.91108 | 39 |
GTATGCC | 12290 | 0.0 | 46.504665 | 45 |
ACCACTC | 23800 | 0.0 | 46.3374 | 32 |